Journal:

Article Title: Phenotypic consequences of lung-specific inducible expression of FGF-3

doi: 10.1073/pnas.101116598

Figure Lengend Snippet: (A) RPA of FGF-3 and GLp65 expression in the lungs after administration of RU486 at a dosage of 500 μg/kg for 1 week (+) on transgenic lines 1292, 1138H, and 1137. Bitransgenic littermates were administered placebo pellets as control (−). RPA of FGF-3 mRNA (B) and Western blot analysis of FGF-3 protein expression (C) in the lungs of mice from line 1138H after administration of placebo or RU486 at a dosage of 66, 130, and 200 μg/kg for 1 month are shown. Each lane represents an independent mRNA and protein from an individual mouse.

Article Snippet: The membranes then were probed with goat anti-mouse FGF-3 polyclonal IgG (Santa Cruz Biotechnology) and horseradish peroxidase-conjugated anti-goat IgG (Santa Cruz Biotechnology).

Techniques: Expressing, Transgenic Assay, Western Blot

Journal:

Article Title: Phenotypic consequences of lung-specific inducible expression of FGF-3

doi: 10.1073/pnas.101116598

Figure Lengend Snippet: Histological changes in the lungs of GLp65/FGF-3 bitransgenic mice treated with placebo or 500 μg/kg RU486. (A) H&E-stained section of lung from placebo-treated mouse. (B) Immunohistochemical staining for Mac-3 of lung from 1137 bitransgenic mouse treated with RU486 for 6 weeks. Free alveolar macrophages (FAMs) are abundant in the alveoli as indicated by the arrow. (C) Immunohistochemical staining for Nkx2.1 of lung from the 1138H bitransgenic mouse treated with 500 μg/kg RU486 for 2 weeks. Type II epithelial cells are increased strikingly along the septa and in alveoli as indicated by the arrow. (D) H&E section of lung from the 1292 bitransgenic mouse treated with 500 μg/kg RU486 for 1 week.

Article Snippet: The membranes then were probed with goat anti-mouse FGF-3 polyclonal IgG (Santa Cruz Biotechnology) and horseradish peroxidase-conjugated anti-goat IgG (Santa Cruz Biotechnology).

Techniques: Staining, Immunohistochemical staining

Journal:

Article Title: Phenotypic consequences of lung-specific inducible expression of FGF-3

doi: 10.1073/pnas.101116598

Figure Lengend Snippet: The response of 1138H bitransgenic mouse lungs to the induction of FGF-3 for 4 weeks with varying doses of RU486. (A) H&E-stained section of lung from placebo-treated 1138H bitransgenic mouse. (B) H&E-stained section of lungs from 1138H bitransgenic mouse treated with 130 μg/kg RU486. FAMs are abundant. (C) H&E-stained section of lungs from 1138H bitransgenic mouse treated with 200 μg/kg RU486. Type II cell hyperplasia is apparent.

Article Snippet: The membranes then were probed with goat anti-mouse FGF-3 polyclonal IgG (Santa Cruz Biotechnology) and horseradish peroxidase-conjugated anti-goat IgG (Santa Cruz Biotechnology).

Techniques: Staining

Journal:

Article Title: Phenotypic consequences of lung-specific inducible expression of FGF-3

doi: 10.1073/pnas.101116598

Figure Lengend Snippet: RPA of FGF-3 (A) and cyclin mRNAs (B) in the lungs of 1138H bitransgenic mouse lungs after the administration and withdrawal of 500 μg/kg RU486. −, RNA from mice treated with placebo; On 1 wk, mice treated with RU486 for 1 week; On 2 wks, mice treated with RU486 for 2 weeks; Off 1 wk, mice after withdrawal of RU486 for 1 week; Off 2 wks, mice after the withdrawal of RU486 for 2 weeks after treatment with RU486; Re-on 2 wks, mice treated with RU486 after withdrawal of RU486 for 2 weeks. Expression of cyclophillin (cph) served as a control for RNA-loading analysis of FGF-3 expression. Expression of L32 served as a control for RNA loading for cyclin gene expression (data not shown). Each lane represents an independent mRNA from an individual mouse.

Article Snippet: The membranes then were probed with goat anti-mouse FGF-3 polyclonal IgG (Santa Cruz Biotechnology) and horseradish peroxidase-conjugated anti-goat IgG (Santa Cruz Biotechnology).

Techniques: Expressing

Journal:

Article Title: Phenotypic consequences of lung-specific inducible expression of FGF-3

doi: 10.1073/pnas.101116598

Figure Lengend Snippet: The effect of FGF-3 induction on IL-7, CM-CSF, and pulmonary epithelial marker gene expression. (A) RPA of the expression of IL-7 and GM-CSF in lungs from 1137 mice treated with 500 μg/kg RU486 for either 1 or 6 weeks. L32 was used as internal control for RNA loading in the RPA. (B) S1 nuclease protection analysis of the expression of epithelial markers, SP-A, SP-B, and SP-C, CCSP, and cytochrome p450 2F2 in bitransgenic mice from line 1138H were administered RU486 for 1 month at a dose of 0, 66, 130, and 200 μg/kg. L32 was used as internal control for RNA loading in the S1 nuclease protection analysis. Control samples were assayed in triplicate (data not shown).

Article Snippet: The membranes then were probed with goat anti-mouse FGF-3 polyclonal IgG (Santa Cruz Biotechnology) and horseradish peroxidase-conjugated anti-goat IgG (Santa Cruz Biotechnology).

Techniques: Marker, Expressing